mycoplasma | Stratech Scientific

Annexin V-FITC kit provides a rapid and convenient assay for apoptosis.Annexin V-FITC kit detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent fluorescein isothiocyante (FITC) dye and necrotic cells using propidium iodide (PI) by flow cytometry and fluorescence microscope. The AnnexinV-FITC kit uses annexin V conjugated with FITC to label phosphatidylserine sites on the membrane surface. The kit also includes propidium iodide (PI) stains necrotic cells with red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.

Biotool™ Annexin V-FITC Apoptosis Detection Kit Protocol

Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

All the steps can be done in the clean bench and the results will show up within 10 mins.

Should the cell culture system be contaminated by mycoplasma, typical metabolic enzymes from mycoplasma will degrade the culture medium components, with typical metabolite products secreted into the cell culture supernatant. The specificity of the metabolites produced by the mycoplasma are very high. Any other type of eukaryotic cells or bacteria will not produce the metabolites.
If the test sample contains the metabolites, the reaction system (including the test sample, the reaction sample well and the reaction buffer) will turn greenish-blue in color. The concentration of metabolites produced is proportional to how dark the color is, which directly indicates the amount of mycoplasma in each sample.

Biotool Mycoplasma Detection Kit – QuickTest